Southampton will host Brentford in the second round of the Carabao Cup on Wednesday 16th September.
The fixture will take place between Saints’ opening two Premier League fixtures away to Crystal Palace and at home to Tottenham, kicking off at 7.45pm BST.
Meeting in the last regionalised round of the competition, it will be the first competitive meeting between the two sides since April 2011.
Coming head-to-head in League One, goals from Adam Lallana, David Connolly and Oscar Gobern secured a 3-0 win for Southampton at Griffin Park.
Brentford, who lost the Championship play-off final last month, remain under the stewardship of Thomas Frank and beat league rivals Wycombe Wanderers on penalties in the first round.