Southampton's youngsters will kick off their 2020/21 EFL Trophy campaign against Stevenage next month, before facing trips to Northampton Town and MK Dons in October and November respectively.
Southampton's youngsters have been drawn in Group C for this season's Trophy, alongside MK Dons and newly promoted Northampton Town of League One and Stevenage of League Two.
Saints are one of 15 invited Premier League teams to compete against tier three and four clubs, doing so for a fifth successive season. As in previous years, Saints will play all group stage fixtures away from home.
Sept 22: Stevenage vs Saints (a) 7:00pm GMT
Oct 6: Northampton Town vs Saints (a) 7:30pm GMT
Nov 10: MK Dons vs Saints (a) 7:30pm GMT