Southampton's youngsters have been drawn alongside AS Monaco, Feyenoord and Derby County in Group F of this season's Premier League International Cup.
The competition consists of six four-team groups, each including two invited international clubs and two Premier League 2 sides.
Saints will play each of their group stage opponents once, with Monaco taking part for the first time, and all matches will be hosted in the UK.
Radhi Jaïdi’s side made it to the final four of last year’s competition, eventually losing out to Dinamo Zagreb in the semi-finals.
Dates and times for fixtures will be announced in due course.