Southampton's youngsters were grouped with Coventry City, Walsall and Forest Green Rovers following last week's EFL Trophy draw, and now dates have been set for each respective Group D (southern section) fixture.
Radhi Jaïdi's side begin this season's competition by travelling to League Two outfit Forest Green Rovers on Tuesday 3rd September, before facing Walsall at Bescot Stadium on Tuesday 1st October.
Their group stage campaign will conclude with a trip to Coventry City, who are playing their 2019/20 home fixtures at Birmingham City's St Andrew's.
As one of 16 invited clubs, all of Saints’ group stage fixtures will take place away from home.
Sept 3: Forest Green Rovers v Saints
The New Lawn, 7pm BST
Oct 5: Walsall v Saints
Bescot Stadium, 7pm BST
Nov 5: Coventry City v Saints
St Andrew's, 7.45pm GMT