Jack Stephens played the full game, while Sam McQueen was an unused substitute, as England Under-21s lost 1-0 away to Germany.
In a result that mirrored that of the senior team on Wednesday night, the Young Lions suffered a narrow friendly defeat
The decisive moment arrived midway through the first half, when Hoffenheim's Nadiem Amiri struck home from the edge of the box.
Stephens had one of the best opportunities to level the match, as the ball fell to him 12 yards out in the 74th minute, but his effort was saved by keeper Julian Pollersbeck.
The defender's Saints teammate, McQueen, was an unused substitute, but will be hoping to see some action when the Under-21s visit Denmark on Monday.