Technical Director Martin Hunter is hoping his side can build on their last Checkatrade Trophy outing when they face Colchester United on Tuesday night.
In their first of three group games, Southampton produced a fine performance to earn a goalless draw at Charlton.
Now, the onus falls on making sure they maintain that level of performance against John McGreal’s side.
Hunter said: “We’ve done our final preparations and we’ve had a good look at Colchester who are a very energetic team so we’re expecting it to be very competitive.
“We need to build on Charlton as it was a good game and the players coped really well with some of their different play.
“You’re playing against men so it’s different from a normal Under-23s game so there are lots of things to work on and be tested.”
Saints dominated proceedings at the Valley, earning a valuable point before losing out on a bonus point on penalties.
And Hunter says being more clinical in front of goal is something that has been discussed in the build-up to this one.
“We have spoken about that in terms of our set-plays and all of work around the penalty areas.
“It’s important and the old adage that the games are won and lost in both boxes is a true one.”